Prep SFC 250

Compact Benchtop Preparative SFC System

Prep SFC 250
Prep SFC 250 MS
Prep SFC 250

Sepiatec’s Prep SFC 250 system is a compact benchtop unit designed for preparative separations using supercritical fluid chromatography.

It measures just 72 x 60 x 88 cm (W x D x H) and offers all the advantages of preparative SFC technology such as fast separations, reduced consumption of organic solvents, lower costs, improved environmental compatibility and increased safety.

The equipment of the Prep SFC 250 system includes:

  • a high pressure 150 ml/min pump for CO2
  • a high pressure 150 ml/min pump for modifier,
  • a back pressure regulator
  • a syringe pump for sample injection
  • a UV / VIS detector
  • a fractionation module with 9 gas/liquid separators for up to 8 fractions + waste
  • an integrated computer with a 15.6" touch-screen
  • the Prep SFC control software
  • optional MS version

Due to its compact design the footprint of the Prep SFC 250 system is comparatively small and precious laboratory space can be saved. It is an excellent complement to other systems for preparative SFC laboratories doing chiral or achiral work. The clearly arranged assembly of the Prep SFC 250 system allows easy access to all of its components.

The fractionation module of the Prep SFC 250 system includes a selector valve and a gas / liquid separator. It is designed for up to 8 sample bottles plus waste with a volume of 1 liter each. Due to the flexible outlets even bigger sample containers with volumes up to several litres can be used.

The user-friendly control software is used via the 15.6" touch screen, which is placed ergonomically on the upper front of the Prep SFC 250 system. A separate computer and a monitor are unnecessary, additional bench space is not required.

As an option Sepiatec’s Prep SFC 250 system can be combined with Advion’s expression CMS. The expression CMS is a very compact mass spectrometer featuring the latest single quadrupole technology. The system as a whole, consisting of Prep SFC 100 and expression CMS, allows users to perform mass-directed-, mass-confirmed and UV-controlled fractionation. The exact molecular weights of the separated substances are quickly and reliably measured, and even UV-inactive targets can be fractionated. The ion source consists of an ESI option and an APCI option, which can both produce anions or cations as required. The acquisition rate is 5,000 m/z units sec-1 and the resolution is 0.5 – 0.7 m/z units (FWHM) at 1,000 m/z units sec-1 throughout the acquisition range.

All the modules needed to connect the expression CMS to the Prep SFC 250 system, such as a makeup flow pump and a split valve, are included. The expression CMS mass spectrometer is operated and the data obtained are displayed using the Prep SFC 100 system software. The expression CMS software allows users to perform a wide range of assessments.The compact size of the Prep SFC 250 and the expression CMS means that they can be installed together in a standard laboratory fume hood.

Subject to modifications.


Operating mode 1 semi-preparative or preparative column
Solvents CO2 and organic solvents
Number of solvents 1 standard, max. 4 solvents with optional selection valve
CO2 pump 150 ml head, 400 bar
Modifier pump 150 ml head, 400 bar
Operating pressure Up to 300 bar, adjustable online backpressure control
Injection Partial loop with syringe pump
Columns 15 to 30 mm ID up to 250 mm length
Column oven Ambient temperature up to 70 °C, 2 columns standard, 6 columns with optional valves
Detection UV / VIS detector 190-500 nm; DAD, ELSD, or mass spectrometer optional
Fraction collection 1 to 8 fractions, unlimited volumes, pressure free collection
Software Prep SFC control software
System Controller Integrated PC, 15.6" TFT touch-screen
Connections 3 USB ports, Ethernet, HDMI
Dimensions 72 x 60 x 88 cm (W x D x H)

Specifications - Advion MS Option

Ion source ESI with APCI option (switchable with minimal effort)
API interface orthogonal ion sampling from heated capillary - allows for small single turbo pump (Patents pending)
Flow rate range 10 µL/min to 500n µL/min (higher with simple split)
Polarity +ve and -ve ion in sequential analyses
m/z range m/z 10 to m/z 1,200
Acquisition rate 5,000 m/z units sec-1 (compatible with UHPLC)
Resolution 0.5 - 0.7 m/z units (FWHM) at 1000 m/z units sec-1 over entire acquisition range
SIM sensivity 10 pg Reserpine (FIA - 5 µL sample injection volume at 100 µL/min) 100:1 S/N (RMS) with SIM of m/z 609.28
Scan sensivity 100 pg Reserpine (FIA - 5 µL sample injection volume at 100 µL/min) 100:1 S/N (RMS) with full-scan acquisition of m/z 100 to m/z 1200
Accuracy ±0.1 m/z units over entire acquisition range