Technical Information for ILS Glass Microsyringes

For more information about glass syringes for HPLC, GC, Automated Liquid Handling, or manual usage, visit our online SYRINGE CATALOG, or Contact Us and request a catalog.

Syringe Quality from ILS

All ILS syringes are manufactured at our manufacturing facility in Germany from high quality DURAN® Borosilicate 33 Type I expansion glass. The 33 expansion glass is the highest chemical resistance and lowest expansion glass that is used for syringe manufacturing and produces the best precision and calibration. All PTFE and metal parts are designed and manufactured in-house using state of the art computer-controlled milling and lathing machines and specialized glass shrinking equipment.

ILS uses a unique process to apply the label markings to the glass barrel. The markings are screen-printed with silver ink and then heated. A chemical exchange occurs between the boron in the glass and the silver to etch the unique brown-colored markings that will not wash off, rub off, or fade over time.

Certificate of Conformance ILS Certificate of Conformance (SAMPLE) Certificate of Calibration ILS Certificate of Calibration (SAMPLE)

syringe marking

Parts of a Syringe & Selecting a Syringe

The glass microsyringe is one of the most commonly used items in science laboratories of all types. There are many different syringe models available to address the hundreds of different applications this precision tool is used for. All syringes begin with a high-quality glass barrel, then the other parts are constructed as needed for each use. The syringe catalog helps to direct you to the specific syringe you need either by type or by application.

Syringe Parts

 

Because ILS produces all parts of all syringes in our own manufacturing facility we are able to manufacture virtually any syringe configuration that you require. You can build your own syringe with the needle, plunger, barrel, and end fittings that will best suit your needs. If you require a customer syringe you may complete the Syringe Questionnaire, or Contact Us for help.

Certificate of Conformance ILS Custom Syringe Questionnaire

 

Using the Syringes

 

Temperature Range and Autoclaving Syringes

 

Syringe Cleaning

After use:

 

Troubleshooting Syringes

 

 

Needle Tip Styles
Needle Description
Needle Tip A

Needle Tip "A"
This tip is designed for optimum penetration of the septa and prevents septa coring. The 17° bevel is the proven standard. In addition our electro tapered tip minimizes the necessary piercing force and diameter of the injection channel. (Cross reference Hamilton 2 or 4 / SGE BV or T)

Needle Tip B

Needle Tip "B"
This tip has a 90° bevel for precise pipetting. This design releases drops completely, for maximum reproducibility. It is widely used for HPLC sample injection. (Cross reference Hamilton 3 / SGE LC)

Needle Tip C

Needle Tip "C"
This tip has been specially designed for autosamplers in routine applications. The electro tapered tip and the polished 90° square end eliminate burrs. It is ideal for PTFE laminated seals and regular septas. (Cross reference Hamilton 2 or AS / SGE C)

Needle Tip D

Needle Tip "D"
Tip "D" is equipped with a side opening for dispensing and filling. This design avoids plugging and minimizes septum damage. It is ideal for thicker septa, recommended for headspace analysis. (Cross reference Hamilton 5 / SGE H)

Gauge Index for ILS Glass Barrel Syringe Needles
Gauge O.D. mm I.D. mm
   33 0.20 0.11
   32 0.25 0.11
   30 0.30 0.15
   29 0.35 0.15
   27 0.40 0.20
   26 0.45 0.25
   26s 0.47 0.13
   25 0.50 0.28
   24 0.55 0.32
   23 0.64 0.35
   23s 0.64 0.15
   22 0.72 0.40
   22s 0.72 0.20
   21 0.80 0.50
   20 0.90 0.60
   19 1.10 0.70
   18 1.30 0.90
   17 1.50 1.10
   16 1.70 1.20
   15 1.80 1.40
   13 2.40 1.90
   12 2.80 2.20
   11 3.00 2.40
   10 3.40 2.70
Needle Types
Type Description
Fixed Needle Fixed Needles. The needle is permanently attached to the glass barrel with glue. Not autoclavable. This design has the least carryover.
Removable Needle Removable Needles. The needle can be removed by unthreading. This allows the needle to be replaced if desired due to damage.
Front Fitting Styles
Type Description
Front Fitting Screw thread 1/4"x28 UNF. Also called "C" and CX" type.
Front Fitting Screw thread 1/4"x28 UNF.
Screw thread M10.
Front Fitting Screw thread M8 x 0.75. Also called "AD" type.
Front Fitting Screw thread M8 x 0.75 with side connection internal threads M6 x 1. . Also called "DAD" type.
Front Fitting Screw thread M10.
Front Fitting Luer connection with side connection internal threads M6 x 1. Also called "D" type.
Front Fitting Luer connection PTFE. Also called "TLL".
Front Fitting Luer connection, glass taper.
Front Fitting Luer connection, PTFE taper.
Front Fitting Tubing connector, available either with Mx or 4x28 threads.

 

Plunger Types
Type Description
Type N
Stainless Steel
Stainless steel plunger for general purpose operation with liquids. Plunger is matched to the barrel and cannot be interchanged.
Type N
Titanium Nickel
Super flexible plunger is very resistant to bending and also resistant to salt buffers. Plunger is matched to the barrel and cannot be interchanged.
Type H
Stainless Steel
Stainless steel plunger with seal attached to the end, suitable for liquids and gases. Also called "gas-tight". The standard seal material is PTFE. Other seal materials may be specified (see below). On most models the seal can be replaced and the plunger can be replaced if necessary.
Type H Plunger Seal Materials
Material Description
PTFE virginal Standard Teflon seal for high chemical resistance. It is low wetting and has good gliding characteristics. It will expand at higher temperatures.
PTFE modified Teflon with lubrication for lower friction and longer lifetime for automatic liquid handling.
PE HD High density polyethylene for better durability with high salt concentration buffers.
PTFE special black New material from ILS has even less friction, less high-temperature expansion, and less electrostatic charge than virgin PTFE and maintains high chemical resistance. It has less cold flow and will provide the highest robustness and longevity. Add "bl" to the part number to order a syringe with the PTFE black seal.
Plunger Top Styles
Type Description
Plunger Top Standard knob.
Plunger Top Similar to the standard knob, but has internal 6-32 UNC threads in the top to allow attachment via a screw. A set screw on the side allows it to be locked.
Plunger Top Has internal 6-32 UNC threads in the top to allow attachment via a screw. A set screw on the side allows it to be locked.
Plunger Top Has internal 6-32 UNC threads in the top to allow attachment via a screw.
Plunger Top Plunger top with mounting hole. Also called "XL" type.
Plunger Top Plunger top with mounting hole and knurled screw.
Plunger Top Tapered plunger top with mounting hole and knurled screw.
Plunger Top Plunger top, ball style. Also called "XE" style.
Plunger Top Plunger top, indented style. Also called "XP" style.

 

Pressure Limits for ILS Glass Barrels
Volume   psi MPa bar
50 ul OD 8 mm 1500 10.8 108
100 ul OD 8 mm 1400 9.7 97
250 ul OD 8 mm 1100 7.7 77
500 ul OD 8 mm 800 5.8 58
1.0 ml OD 8 mm 500 3.7 37
2.5 ml Wall thickness 1.5 mm 300 2.3 23
5.0 ml Wall thickness 1.5 mm 200 1.7 17
10.0 ml Wall thickness 1.5 mm 180 1.3 13
25.0 ml Wall thickness 2.0 mm 150 1.1 11
50.0 ml Wall thickness 2.0 mm 115 0.8 8
100.0 ml Wall thickness 2.0 mm 115 0.8 8